Composite
Part:BBa_K2933260:Design
Designed by: Ruoming Sun Group: iGEM19_TJUSLS_China (2019-09-15)
RBS b+Linker h+His+Linker a+Sumo+Linker b+ARL-1+T7 terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 298
Illegal PstI site found at 824
Illegal PstI site found at 938
Illegal PstI site found at 989 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 298
Illegal NheI site found at 75
Illegal NheI site found at 1254
Illegal PstI site found at 824
Illegal PstI site found at 938
Illegal PstI site found at 989 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 298
Illegal BglII site found at 187
Illegal BamHI site found at 386 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 298
Illegal PstI site found at 824
Illegal PstI site found at 938
Illegal PstI site found at 989 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 298
Illegal PstI site found at 824
Illegal PstI site found at 938
Illegal PstI site found at 989 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
NCBI.