Composite

Part:BBa_K2933260:Design

Designed by: Ruoming Sun   Group: iGEM19_TJUSLS_China   (2019-09-15)


RBS b+Linker h+His+Linker a+Sumo+Linker b+ARL-1+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 824
    Illegal PstI site found at 938
    Illegal PstI site found at 989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
    Illegal NheI site found at 75
    Illegal NheI site found at 1254
    Illegal PstI site found at 824
    Illegal PstI site found at 938
    Illegal PstI site found at 989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
    Illegal BglII site found at 187
    Illegal BamHI site found at 386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 824
    Illegal PstI site found at 938
    Illegal PstI site found at 989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 824
    Illegal PstI site found at 938
    Illegal PstI site found at 989
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

NCBI.

References